Antinociceptive and anti-inflammatory activities of the ethanolic extract, of fractions and of epicatechin isolated from the stem bark of Ximenia americana L. (Oleacaceae)

Valdiléia Teixeira Uchôa, Thays de Lima Matos Freire Dias, Gabriela Muniz de Albuquerque Melo, Yolanda Karla Cupertino Da Silva, Aline Cavalcanti Queiroz, Henrique Fonseca Goulart, Magna Suzana Alexandre-Moreira, Antonio Euzébio Goulart Sant´Ana

Resumo


The antinociceptive and anti-inflammatory actions of the ethanolic extract, fractions and epicatechin (XM-Catechin) isolated from the ethanoic extracts of bark of Ximenia americana L. were determined utilizing in vivo models such as acetic acid-induced writhing, the formalin test, hot plate test, zymosan-induced peritonitis and in vitro cyclooxygenase inhibition assay. The writhing test revealed inhibitory effects. The formalin test demonstrated an antinociceptive effect in both early and late phases by XM-catechin, while the chloroform fraction showed a lower antinociceptive effect at the early phase. The aqueous fraction and ethanolic extract caused significant inhibition at the late phase. Treatment of mice with XM-catechin or with the ethyl acetate fraction had no central antinociceptive effect in the hot plate test. Anti-inflammatory effects were determined for zymosan-induced peritoneal inflammation and the data indicated that XM-catechin, the hydromethanol fraction, the ethanol extract and the ethyl acetate fraction reduced the number of recruited cells. These results demonstrate that the extract, fractions and XM-Catechin produce antinociceptive and anti-inflammatory responses. According to the results obtained in the cyclooxygenase inhibition assays, the observed effects are related to inhibition of COX-1 and COX-2. The biological actions demonstrated in the present study support the ethnomedicinal use of this plant.

Palavras-chave


Medicinal plants, anti-inflammatory drugs, corticosteroids, cyclooxygenase



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