Extraction of Pectin in Seriguela (Spondias Purpurea L.) Using Experiments Designs: a Tutorial Using the Free Software ‘’R’’

Resumo

This manuscript describes an experiment that emphasizes concepts related to the design of experiments
using the computational environment R, which can be used by beginners, undergraduate students and
postgraduate researchers. The pectin extraction experiment using seriguela bark (Spondias purpurea L.)
was chosen because most of the necessary materials are available in analytical laboratories. Additionally,
this tutorial provides an easy guide for installing R and other dependencies. The approach used in this
tutorial introduces multivariate concepts, both in screening and in experimental optimization. First, a
fractional factorial design is applied and the model is evaluated to define the experimental domain. Second,
an extension of the factorial design is described, thus providing a Box-Behnken design for experimental
optimization. The quadratic model is then introduced and used to construct the response surface.