Otimização do Extrato Bruto Hidroetanólico das Folhas de Eugenia dysenterica DC. pelo Método Box‑Behnken e Avaliação das suas Atividades Antioxidante e Antimicrobiana





Eugenia dysenterica DC., “cagaita”, is a tree whose leaves are popularly used for wounds, diarrhea and
dysentery. The aim of this study was to obtain the standardized crude hydroethanolic extract from E.
dysenterica leaves and evaluate the antioxidant and antimicrobial activities. Quality control was carried
out using conventional techniques, extract elevated by Box-Behnken, antioxidant activity by DPPH and
ABTS and antimicrobial activity by broth microdilution. The extract with the highest concentration of
tannins was obtained with 60% hydrohydroethanolic solution, plant material/solvent ratio of 12 mg mL-1.
The concentration of tannins in the extract was 1.71 g TAE g-1, phenols 6.23 mg GAE mg-1 and flavonoids
4.70 mg of rutin per mg. The standardized crude hydroethanolic extract showed excellent antioxidant
activity both by DPPH (IC50 of 2.70 ± 0.005 μg mL-1) and by ABTS (3479.21 ± 0.001 μM trolox g-1).
Moderate antifungal activity of the standardized hydroethanolic extract was verified against C. gattii
and C. neoformans. This study represents the first standardization of the crude hydroethanolic extract of
E. dysenterica that demonstrated high antioxidant activity. This species has antifungal potential, which
encourages further studies.