Carvacryl acetate: synthesis and toxicological and pharmacological activities

Resumo

In this work, we report a synthetic route to carvacryl acetate. In addition to the chemical synthesis, this study aimed to evaluate the acute toxicity after administration of carvacryl acetate at doses of 1000 and 2000 mg/kg in male and female Swiss mice treated orally and intraperitoneally. Carvacryl acetate was aseptically suspended in 0.05% Tween 80, dissolved in 0.9% saline (vehicle) and administered orally (p.o.) and intraperitoneally (i.p.) (0, 1000 and 2000 mg/kg). In addition to the acute toxicity, the present study evaluated the antioxidant capacity by inhibition of oxidative hemolysis and using DPPH•, ABTS•+ and by reducing potential. Signs of toxicity and mortality were observed in animals of both sexes after intraperitoneal administration of carvacryl acetate (2000 mg/kg). Additionally, no significant difference in body weight, water intake, food consumption and excreta production of treated animals were detected in comparison with control group (vehicle). In vitro study demonstrated antioxidant capacity using DPPH• (EC50 = 6.1 ± 0.53 µg/ml), ABTS•+ (EC50=5.24 ± 0,38 µg/ml), reducing potential (EC50=3.65 µg/ml) and by inhibition of oxidative hemolysis induced by hydrogen peroxide (EC50= 2.58 ± 0,07 µg/ml). In summary, this study indicated that treatment with carvacryl acetate in selected doses was well tolerated in all animals.