Cytotoxicity assessment of Siparuna cymosa essential oil in the presence of myeloid leukemia cells

Autores

  • Rafaela Oliveira da Silva Universidade Estadual de Santa Cruz
  • Fernanda Cristina Gontijo Evangelista Universidade Federal de Minas Gerais
  • Adriano de Paula Sabino Universidade Federal de Minas Gerais
  • Luiz Alberto Mattos da Silva Universidade Estadual de Santa Cruz
  • Fernando Faustino de Oliveira Universidade Estadual de Santa Cruz
  • Rosilene Aparecida de Oliveira Universidade Estadual de Santa Cruz http://orcid.org/0000-0002-4210-7215

Palavras-chave:

Química de Produtos Naturais, óleos essenciais,

Resumo

This work presents the chemical characterization of the essential oil obtained from the Siparuna cymosa (Siparunaceae), an endemic species of the Atlantic Rainforest, as well as its cytotoxicity assessment in the presence of tumor cells. The oil was extracted from dry leaves by hydrodistillation and characterized (GC-MS); and its cytotoxicity was assessed by 3-(4,5-dimethyl-2-thyazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric method in AML, Acute Myeloid Leukemia (THP-1) and CML, Chronic Myeloid Leukemia (K652) cell lines. The average oil content was 2.11%, and the majority compound was a-bisabolol (68.9%) and, in significant amounts, p-cimen-9-ol (7.9%) and spathulenol (3.7%). The IC50 obtained were the 25.44 ± 1.55 μg mL-1 (CML), and 30.88 ± 2.45 μg mL-1 (AML). The essential oil exhibits moderate cytotoxic action in the presence of leukemia cells, especially AML, for which the selectivity index was higher. The oil's cytotoxic action in the presence of the essential oil’s antitumor cells may be associated with the presence of the component a-bisabolol, a substance with recognized antitumor action.

Keywords: Siparunaceae; GC-MS analysis; bisabolol; THP-1; K562

Biografia do Autor

Rosilene Aparecida de Oliveira, Universidade Estadual de Santa Cruz

Professora do Departamento de Ciências Exatas e Tecnologicas da Universidade Estadual de Santa Cruz, Bahia. Pesquisadora na área Química de Produtos Naturais.

Publicado

21-12-2020